More sensitive and faster instrumentation along with tools for multiplexing have greatly increased the throughput of proteomic analysis. To gain a deeper understanding of cell signaling events involved in oncogenic transformation, we are taking advantage of this increased throughput to perform multiple, complementary proteomic experiments on cells undergoing the epithelial to mesenchymal transition (EMT). EMT is a fundamental process in early embryonic development that also is implicated in tumor metastasis. In addition to monitoring global protein abundance and changes in protein phosphorylation as cells transit through EMT, we are also developing and adapting methods for tracking sub-cellular localization and changes in protein interactions during this process.
Dr. Dephoure is an Assistant Professor at the Sandra and Edward Meyer Cancer Center at Weill Cornell Medical College. His lab develops and refines proteomic tools and uses them to study signaling events that underlie basic cellular biology and human disease research. A long-term goal of the lab is to understand the complex roles of posttranslational protein modifications (PTMs) in cellular growth and proliferation. By combining multiplexed quantitative and temporal analysis of PTMs with novel methods for studying changes in protein interactions and subcellular localization, they are decoding the mechanisms by which these simple chemical changes impact oncogenesis and tumor progression.